کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2463445 | 1555121 | 2006 | 6 صفحه PDF | دانلود رایگان |
The chemotaxis of porcine peripheral blood polymorphonuclear cells (PMN) was enhanced by culture supernatant from peripheral blood mononuclear cells (PBMC) treated with trans10–cis12 (10t–12c) conjugated linoleic acid (CLA) or by porcine recombinant (pr) interleukin (IL)-8. To identify this chemotactic inducing factor, the culture supernatant from 10t–12c CLA treated PBMC was partially purified by gel filtration. Only fraction 9 contained enhanced PMN chemotactic activity. The chemotactic activity of fraction 9 was inhibited by an anti-prIL-8 polyclonal antibody (pAb). Porcine IL-8 mRNA expression analyzed by a reverse transcriptase-polymerase chain reaction (RT-PCR) assay was increased in PBMC by the addition of 10t–12c CLA. This study strongly suggests that the immunoenhancing effect of CLA on the chemotactic response of porcine PMN is mediated through IL-8 produced by CLA treated PBMC.
Journal: Veterinary Immunology and Immunopathology - Volume 112, Issues 3–4, 15 August 2006, Pages 284–289