کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2478728 1556021 2011 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Simultaneous HPLC–UV analysis of telmisartan and hydrochlorothiazide in human plasma
موضوعات مرتبط
علوم پزشکی و سلامت داروسازی، سم شناسی و علوم دارویی اکتشاف دارویی
پیش نمایش صفحه اول مقاله
Simultaneous HPLC–UV analysis of telmisartan and hydrochlorothiazide in human plasma
چکیده انگلیسی

A specific, sensitive and rapid method based on high performance liquid chromatography (HPLC) was developed for the simultaneous determination of telmisartan (TELM) and hydrochlorothiazide (HCT) in human plasma using indapamide as internal standard. The method utilizes proteins precipitation with acetonitrile as only sample preparation prior to RP-HPLC.The analytes were chromatographed on shim-pack cyanopropyl column in isocratic elution with methanol: 10 mM ammonium acetate solution (pH 6.0) (35:65 v/v) as mobile phase at a flow rate of 1 ml/min and the wavelength of detection was 270 nm.The method was validated over the concentration range of 1–10 μg ml−1 for TELM and 0.31–3.12 μg ml−1 for HCT in human plasma.Inter- and intra-run precision of TELM and HCT were less than 3.60% and the accuracy was less than 1.868%. The linearity, recovery, matrix effect and stability were validated for TELM/HCT in human plasma.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bulletin of Faculty of Pharmacy, Cairo University - Volume 49, Issue 1, June 2011, Pages 19–24
نویسندگان
,