کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2480978 1556228 2011 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Encapsulation of Aβ1–15 in PLGA microparticles enhances serum antibody response in mice immunized by subcutaneous and intranasal routes
موضوعات مرتبط
علوم پزشکی و سلامت داروسازی، سم شناسی و علوم دارویی اکتشاف دارویی
پیش نمایش صفحه اول مقاله
Encapsulation of Aβ1–15 in PLGA microparticles enhances serum antibody response in mice immunized by subcutaneous and intranasal routes
چکیده انگلیسی

The aim of the present work was to develop an easy, safe and effective vaccine in Balb/c mice using the Aβ1–15 peptide as immunogen entrapped in PLGA microparticles to reduce the risk of an adverse T cell-mediated response. Aβ1–15, which contains the N-terminal antibody epitope of the full Aβ1–42 peptide was encapsulated in PLGA by a modified solvent evaporation/extraction technique using a double emulsion system. Microparticles were characterized in terms of size distribution (1.22 ± 0.28 μm), encapsulation efficiency (75.05 ± 4.17%), surface associated peptide (59.81 ± 0.96%) and “in vitro” release profile. Balb/c mice were immunized by subcutaneous and intranasal routes with three 30 μg doses of the peptide microencapsulated in PLGA. A solution of the peptide alone and an emulsion in the Freund’s adjuvant were administered subcutaneously as control groups. Antibody levels elicited against the toxic Aβ1–40 fraction in the serum of PLGA microparticles treated groups were higher than that of the peptide alone groups. Our initial results indicate that immunotherapy with Aβ1–15 loaded PLGA microparticles could be a promising approach for the future development of a safe vaccine against Alzheimer’s disease.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Pharmaceutical Sciences - Volume 44, Issue 3, 9 October 2011, Pages 200–206
نویسندگان
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