کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2496325 1116122 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Potent anti-angiogenic and cytotoxic effect of conferone on human colorectal adenocarcinoma HT-29 cells
ترجمه فارسی عنوان
اثر آنتی رگ زایی و سیتوتوکسی قوی کانفرون بر سلولهای HT-29 آدنوکارسینوم روده بزرگ انسانی
کلمات کلیدی
کانفرون؛ P-گلیکوپروتئین؛ Angiopoietin-1؛ گونه های اکسیژن واکنش پذیر؛ آدنوکارسینوم کولورکتال P-gp، P-glycoprotein؛ VEGF، فاکتور رشد اندوتلیال عروقی؛ Ang-1، angiopoietin-1؛ Ang-2، angiopoietin-2؛ EC، سلول اندوتلیال؛ VEGFR-1، - 2، اندوتیک عروقی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی بالینی
چکیده انگلیسی

BackgroundCancer is one of the leading causes of death worldwide, both in developed and developing countries. Of note, colorectal adenoma encompasses a high rate of gastrointestinal-associated cancer death in human being. Today, different strategies, including surgery approaches, photodynamic therapy, radiation and particularly natural compounds have been extensively used to manage tumor behavior in human body.MethodsThe objective of the present study was to elucidate the multilateral effects of conferone on HT-29 cell lines. In addition to cell cytotoxicity, the extent of lipid peroxidation, MDA formation, catalase, superoxide dismutase and intracellular ROS levels, as markers of oxidative stress, were also studied. P-glycoprotein-mediated cellular efflux effectiveness, anti-angiogenic and finally anti-migratory capacities of conferone-exposed HT-29 cells were monitored over a course of 72 h.ResultsIt was found that, conferone mediated cell proliferation arrest and induced cell death through both apoptosis and necrosis phenomena. HT-29 cells, exposed to 20 µM conferone, under gone oxidative stress and total content of reactive oxygen species was increased in a time-dependent manner. Intracellular accumulation of rhodamine 123 and cell's swelling under iso- and hypo-osmotic conditions could be related to P-glycoprotein incorrect performance in the presence of conferone. A significant reduction in CD31 positive cells population and in vitro tubulogenesis of endothelial cells was also observed after incubation with conditioned medium collected from 72 h conferone-treated HT-29 cells. Conferone also precluded angiogenesis capability of treated HT-29 cells through an altered secretome profile, including vascular endothelial growth factor, Angiopoietin-1 and -2 factors. In addition to anti-angiogenic properties of conferone, a profound decrease in migration capability of HT-29 cells was also evident.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Phytomedicine - Volume 23, Issue 4, 15 April 2016, Pages 398–405
نویسندگان
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