کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2496858 1116170 2012 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
In vitro anti-influenza virus activity of a cardiotonic glycoside from Adenium obesum (Forssk.)
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی بالینی
پیش نمایش صفحه اول مقاله
In vitro anti-influenza virus activity of a cardiotonic glycoside from Adenium obesum (Forssk.)
چکیده انگلیسی

Methanolic extracts of six Saudi plants were screened for their in vitro antiviral activity using influenza virus A/PR/8/34 (H1N1) and MDCK cells in an MTT assay. The results indicated that the extracts of Adeniumobesum and Tephorosianubica possessed antiviral activity (99.3 and 93.3% inhibition at the concentration of 10 μg/ml, respectively). Based on these results A. obesum was selected for further study by applying bioactivity-guided fractionation to isolate its antiviral principle. The antiviral principle was isolated from the chloroform fraction through solvent fractionation, combined open liquid chromatography and HPLC. The isolated active compound A was identified as oleandrigenin-β-d-glucosyl (1 → 4)-β-d-digitalose, on the basis of its spectral analysis (MS, 1D and 2D NMR). The isolated glycoside showed reduction of virus titre by 69.3% inhibition at concentration of 1 μg/ml (IC50 = 0.86 μg/ml).

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Phytomedicine - Volume 19, Issue 2, 15 January 2012, Pages 111–114
نویسندگان
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