کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2497307 | 1116196 | 2009 | 6 صفحه PDF | دانلود رایگان |

This study aimed to investigate the effects of obovatol isolated from Magnolia obovata on pentobarbital-induced sleeping behaviors and to determine whether these effects were mediated by GABAA receptors/chloride channel activation, using a western blot technique and Cl− sensitive fluorescence probe. GABAA receptors subunits expression and chloride influx were investigated in cultured cerebellar granule cells. Obovatol (0.05, 0.1, and 0.2 mg/kg) prolonged the sleeping time induced by pentobarbital (42 mg/kg). In addition, obovatol (20 and 50 μM) significantly increased Cl− influx in the primary cultured cerebellar granule cells. Moreover, obovatol increased the expression of GABAA receptor α-, β-, and γ-subunits. However, it had no effect on the abundance of the expression of glutamic acid decarboxylase (GAD), suggesting that obovatol might not activate GAD. These results suggest that obovatol potentiates pentobarbital-induced sleeping time through the GABAA receptors/chloride channel activation.
Journal: Phytomedicine - Volume 16, Issue 4, April 2009, Pages 308–313