Immunomodulatory and antioxidants properties of fixed combination of fish oil with plant extracts

• Immunomodulatory and antioxidant properties of fixed combination of fish oil with schisandra oil extract (CFS) and chamomilla oil extract (CFM) were evaluated in vivo and in vitro.
• The fixed combinations exhibited inhibition of the delayed-type hypersensitivity in mice.
• CFM showed stimulation of humoral immunity and increase of secondary antibody titer.
• Combination of fish oil with plant extracts lead to significant synergistic antioxidant effect.

The immunomodulatory and antioxidant properties of fixed combination of fish oil with Schisandra chinensis oil extract (CFS) and Matricaria chamomilla oil extract (CFM) (5 volumes of fish oil with 1 volume of plant extract) were evaluated in vivo and in vitro. The fixed combinations exhibited 27.8% (CFS) and 21.7% (CFM) inhibition of the delayed-type hypersensitivity in mice at 5460 mg/kg dose which was greater than that of fish oil (16.2%) along. Also CFM at 5460 mg/kg showed statistically significant (p < 0.05) stimulation of humoral immunity and increase of secondary antibody titer (5.3 ± 0.2) compared with the control (4.3 ± 0.2). The LD50 of both combinations was estimated to be greater than 5000 mg/kg since there were no lethality and signs of acute intoxication after 48 h observation. Fish oil showed weak efficacy toward scavenging of 1,1-diphenyl-2-picrylhydrazyl free radicals (IC50 = 250.2 ± 5.0 mg/kg), while combination of fish oil with plant extracts lead to significant synergistic antioxidant effect with IC50 = 119.1 ± 3.4 mg/kg for CFS and 49.4 ± 1.2 mg/kg for CFM. Overall, data presented here are important as a sign that fixed combinations of fish oil with chamomile and schisandra oil extracts substantially contributes to the immunomodulatory, as well as to the antioxidant activity.

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