Evaluation of lipofuscin-like pigments as an index of lead-induced oxidative damage in the brain

The results show that the concentration of LFP in rat brain homogenates increased significantly after 2 h incubation with 10−2 M lead acetate as compared to controls (P < 0.0001). This effect was not observed in lower doses of lead acetate (10−4 and 10−6 M). After the long-term exposure of mice to 0.2% lead acetate, pronounced accumulation of lead and significantly increased concentration of LFP (P < 0.004) in the brains of exposed animals as compared to controls were observed. The evidence for the formation of specific fluorophores originating from oxidative damage was shown also in qualitative changes in 3D spectral arrays and synchronous spectra. The presented results proved the influence of lead on the activation of radical reactions in the brain after short in vitro exposure of rat brain as well as within long-term in vivo exposure in mice using lipofuscin-like pigments as an indicator of oxidative stress.