کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2507327 1117484 2015 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development and validation of a high-performance thin layer chromatography method for the determination of cholesterol concentration
ترجمه فارسی عنوان
توسعه و اعتبار سنجی یک روش کروماتوگرافی نازک با عملکرد بالا برای تعیین غلظت کلسترول
کلمات کلیدی
کلسترول، تجزیه و تحلیل دزیتومتریک، زرده تخم مرغ، روش کار با کروماتوگرافی نازک با کارایی بالا
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
چکیده انگلیسی

An accurate, sensitive, precise, reliable, and quick method for the determination of cholesterol content by high-performance thin layer chromatography is developed. In this method, aluminum-backed precoated silica gel 60 F254 plates were used as the stationary phase and the samples were sprayed with the help of CAMAG sample applicator Linomat 5. The chromatogram was developed with the mobile phase consisting of chloroform:methanol (9.5:0.5, v/v). The samples were detected using CAMAG Scanner 4 and evaluated using the method developed on winCATS software. Densitometric analysis of cholesterol was performed in absorbance mode at 200 nm. In this solvent system, cholesterol gave a compact spot with an Rf value of 0.63 ± 0.03. The linear regression analysis of data for the calibration curve showed good linearity over a concentration range of 2–7 μg/spot with a regression value of 0.99933 and standard deviation of 1.44%. The limit of detection and limit of quantification were found to be 100 ng/spot and 500 ng/spot, respectively. Using the developed method, the concentration of cholesterol in the saponified and unsaponified egg yolk sample was determined. This method was found to be reproducible and can even be used for samples containing complex matrices.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Food and Drug Analysis - Volume 23, Issue 2, June 2015, Pages 219–224
نویسندگان
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