کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2532843 | 1559027 | 2011 | 9 صفحه PDF | دانلود رایگان |
The purpose of the present study was to determine the relationship between the responses of transient and sustained K+ currents, and action potentials to ouabain, and to compare the immunoreactive expression of alpha Na+–K+-ATPase isoforms (α1, α2 and α3) in neonatal rat small-diameter nodose ganglion neurons. We used perforated patch-clamp techniques. We first confirmed that the neurons (n = 20) were insensitive to 0.5 μM tetrodotoxin (TTX). Application of 1 μM ouabain 1) decreased the transient K+ currents in 60% of neurons and the sustained K+ currents in 20%, 2) increased voltage-gated transient and sustained K+ currents in 20% of neurons, and 3) had no effect on transient K+ currents in 20% of neurons and on sustained K+ currents in 60%. Thirteen of the neurons were of a rapidly adapting type, and the remaining 7 were of a slowly adapting type. In 6 rapidly adapting type neurons (46%), their activity was not significantly altered by ouabain application, but in 4 rapidly adapting type neurons, the activity increased. In the remaining 3 rapidly adapting type neurons, ouabain application hyperpolarized the resting membrane potential. The slowly adapting type 7 neurons each showed increased activity after 1 μM ouabain application. The α1 isoform of Na+–K+-ATPase was identified as the predominant immunoreactive isoforms in small-diameter nodose ganglion neurons. These results suggest that the increased activity of small-diameter nodose ganglion neurons seen after application of 1 μM ouabain is mediated by direct inhibition of the transient K+ current.
Journal: European Journal of Pharmacology - Volume 659, Issues 2–3, 1 June 2011, Pages 130–138