کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2533617 1559058 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effect of the anti-diabetic drug metformin on bone mass in ovariectomized rats
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب سلولی و مولکولی
پیش نمایش صفحه اول مقاله
Effect of the anti-diabetic drug metformin on bone mass in ovariectomized rats
چکیده انگلیسی

Recent studies have reported bone loss in the patients with diabetes and a direct osteogenic effect of metformin on osteoblast-like cells in culture. In this study, we investigated the action of metformin on bone mass in ovariectomized (OVX) rats. Three months after either a sham surgery or bilateral ovariectomy, thirty-two female Sprague–Dawley rats were randomly assigned into four groups: (1) Sham group; (2) OVX group; (3) OVX + metformin (50 mg/kg/day) group; and (4) OVX + metformin (100 mg/kg/day) group. After 2 months of oral administration with or without metformin, tibiae were harvested for dual energy X-ray absorptiometry, micro-computed tomography (micro-CT) and histology analysis, while the bone marrow cells from tibiae were collected for measurement of the mRNAs expressions for three osteoblast genes and estrogen receptors alpha by the use of real-time RT-PCR. We found that the impaired bone density and quality induced by bilateral ovariectomy were significantly improved by the treatment of metformin (both 50 and 100 mg/kg/day), and this action could be partly mediated by regulating bone marrow cells development through induction of mechanisms regulating osteoblast markers core binding factor a1 and LDL receptor-related protein 5. These findings provide new evidence that the anti-diabetic drug metformin has a direct inhibition effect on bone loss in OVX rats, in addition to its well-documented osteogenic potency in vitro.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Pharmacology - Volume 635, Issues 1–3, 10 June 2010, Pages 231–236
نویسندگان
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