کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2534443 1559088 2009 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
CTN-986, a compound extracted from cottonseeds, increases cell proliferation in hippocampus in vivo and in cultured neural progenitor cells in vitro
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب سلولی و مولکولی
پیش نمایش صفحه اول مقاله
CTN-986, a compound extracted from cottonseeds, increases cell proliferation in hippocampus in vivo and in cultured neural progenitor cells in vitro
چکیده انگلیسی

We have previously reported that Quercetin 3-O-b-d-apiofuranosyl-(1→2)-[a-l-rhamnopyranosyl-(1→6)]-b-d-glucopyranoside (CTN-986), a potential antidepressant extracted from glandless cottonseeds, exerted a notable antidepressant-like effect in experimental animal models. Recent evidence suggested, at least in some animal models, that the behavioral effects of chronic antidepressant treatment were mediated by the stimulation of hippocampal neurogenesis. To explore possible mechanisms of CTN-986′s antidepressant-like effect, CTN-986 (10 mg/kg, i.g) or imipramine (IMI, 10 mg/kg, i.g) was administered once per day to the chronically stressed mice over 21 days. Immunohistochemical analysis revealed that chronic CTN-986 treatment increased bromodeoxyuridine (BrdU; thymidine analog as a marker for dividing cells)-positive cells in the hippocampal dentate gyrus in stressed mice, as was the case with chronic IMI treatment. Moreover, using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay and [3H] thymidine incorporation assay, it was found that exposure to CTN-986 for 4 days dose-dependently increased the proliferation of the cultured hippocampal neural progenitor cells (NPCs). This effect was significantly reversed by co-incubation with serotonin 1A (5-HT1A) receptor antagonist WAY100635. These results indicate that CTN-986 increase hippocampal NPCs proliferation which might be closely related to the activation of the 5-HT1A receptor. This finding can shed light on the mechanisms for its antidepressant-like effects.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Pharmacology - Volume 607, Issues 1–3, 1 April 2009, Pages 110–113
نویسندگان
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