کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2535187 1559108 2008 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
GSK-3β inhibition and prevention of mitochondrial apoptosis inducing factor release are not involved in the antioxidant properties of SB-415286
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب سلولی و مولکولی
پیش نمایش صفحه اول مقاله
GSK-3β inhibition and prevention of mitochondrial apoptosis inducing factor release are not involved in the antioxidant properties of SB-415286
چکیده انگلیسی

The antioxidant effects of lithium and SB-415286, two glycogen synthase kinase-3β (GSK-3β) inhibitors, were studied in cerebellar granule neurons by measuring changes in 2, 7-dichlorodihydrofluorescein diacetate (H2DCFDA) fluorescence. GSK-3β inhibitors inhibit apoptosis mediated by serum and potassium withdrawal (S/K withdrawal) and GSK-3β activation, as measured by β-catenin degradation. Furthermore, as both drugs prevent mitochondrial apoptosis inducing factor (AIF) release, these data indicate that GSK-3β inhibitors prevent caspase-independent apoptosis in cerebellar granule neurons induced by S/K withdrawal. While the most specific GSK-3β inhibitor, SB-415286, demonstrated antioxidant effects, Li+ 10 mM did not. These results indicate that lithium 10 mM and SB-415286 20 µM exert anti-apoptotic effects in cases of S/K withdrawal mediated by GSK-3β inhibition. However, these antioxidant properties are independent of GSK-3β inhibition and prevention of mitochondrial AIF release.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Pharmacology - Volume 588, Issues 2–3, 7 July 2008, Pages 239–243
نویسندگان
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