کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2535240 1559111 2008 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Involvement of NF-κB and AP-1 activation in icariin promoted cardiac differentiation of mouse embryonic stem cells
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب سلولی و مولکولی
پیش نمایش صفحه اول مقاله
Involvement of NF-κB and AP-1 activation in icariin promoted cardiac differentiation of mouse embryonic stem cells
چکیده انگلیسی

Icariin has been reported to facilitate the differentiation of mouse embryonic stem (ES) cells into cardiomyocytes; however, the mechanism on cardiomyogenic cell lineage differentiation has not been fully elucidated yet. In the present studies, an underlying signaling network including p38, extracellular signal-regulated kinase 1, 2 (ERK1, 2), nuclear factor-κB (NF-κB), activator protein-1 (AP-1) transcription factors c-jun and c-fos was assumed in icariin induced cardiomyogenesis. Icariin rapidly activated p38 and ERK1, 2 in embryoid bodies, treatment with p38 antagonist 4-(4-Fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-1H-imidazole (SB203580) or ERK1, 2 inhibitor 1,4-diamino-2,3-dicyano-1,4-bis[2-aminophenylthio] butadiene (U0126) significantly abolished icariin induced cardiac commitment, MEF2C gene expression and nuclear translocation, as well as cardiac-specific protein α-actinin expression, indicating that p38 and ERK1, 2 are specifically involved in icariin stimulated cardiomyogenic cell lineage differentiation of ES cells. Further, IκBα phosphorylation and NF-κB p65 translocation to the nucleus appeared rapidly when embryoid bodies exposed to icariin, and the expression of IκBα or NF-κB p65 in cytoplasm was decreased concomitantly. Moreover, icariin increased c-jun and c-fos mRNA and protein expression. Either SB203580 or U0126 displayed inhibitory effect on icariin induced NF-κB and AP-1 activation. It could be concluded that p38 and ERK1, 2 are activated in a coordinated manner, which in turn contribute to NF-κB and AP-1 activation in icariin induced cardiomyogenic cell lineage differentiation of mouse ES cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Pharmacology - Volume 586, Issues 1–3, 31 May 2008, Pages 59–66
نویسندگان
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