کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2535431 | 1559119 | 2008 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Mu-opioid receptor heterooligomer formation with the dopamine D1 receptor as directly visualized in living cells Mu-opioid receptor heterooligomer formation with the dopamine D1 receptor as directly visualized in living cells](/preview/png/2535431.png)
Our immunohistochemistry experiments demonstrated that the mu-opioid receptor co-localized with the dopamine D1 receptor in neurons of the cortex and caudate nucleus. On the basis of this physiological data we further investigated whether these two G protein coupled receptors formed hetero-oligomers in living cells. To demonstrate hetero-oligomerization we used a novel strategy, the method used harnessed the physiological cellular mechanism for transport of proteins to the nucleus. The nuclear translocation pathway was adapted for the visualization of mu-opioid hetero-oligomers with the dopamine D1 receptor. The receptor hetero-oligomer complex formed resulted in a significantly enhanced surface expression of mu-opioid receptor. This hetero-oligomer formation involved the interaction of mu-opioid receptor with the dopamine D1 receptor carboxyl tail, since a dopamine D1 receptor substituted with the carboxyl of the dopamine D5 receptor failed to increase surface expression of mu-opioid receptor.
Journal: European Journal of Pharmacology - Volume 581, Issue 3, 10 March 2008, Pages 235–243