کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2536345 1559146 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Acetic acid opens large-conductance Ca2+-activated K+ channels in guinea pig detrusor smooth muscle cells
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب سلولی و مولکولی
پیش نمایش صفحه اول مقاله
Acetic acid opens large-conductance Ca2+-activated K+ channels in guinea pig detrusor smooth muscle cells
چکیده انگلیسی

Acetic acid was found to have actions on urinary bladder smooth muscle in our routine ion channel screening assays. Numerous studies have examined the mechanisms of bladder irritation by acetic acid; however, the direct effect of acetic acid on ion channels in detrusor smooth muscle cells has not been evaluated. We used whole-cell patch-clamp techniques to examine the effect of acetic acid on large-conductance Ca2+-activated K+ channels (BKCa) from guinea pig detrusor smooth muscle cells and CHO cells expressing recombinant human BKCaαβ1 (CHO BKCaαβ1) and human BKCaα (CHO BKCaα). Acetic acid activated BKCa currents in a concentration-dependent (0.01% to 0.05% v/v) manner in all the cell systems studied. Acetic acid (0.05%) increased BKCa current at + 30 mV by 2764 ± 918% (n = 8) in guinea pig detrusor smooth muscle cells. Acetic acid (0.03%) shifted the V1/2 of conductance–voltage curve by 64 ± 14 (n = 5), 128 ± 14 (n = 5), and 126 ± 12 mV (n = 4) in CHO BKCaα, CHO BKCaαβ1 and detrusor smooth muscle cells, respectively. This effect of acetic acid was found to be independent of pH and was also not produced by its salt form, sodium acetate. Automated patch-clamp experiments also showed similar activation of CHO BKCaαβ1 by acetic acid. In conclusion, acetic acid directly activates BKCa channels in detrusor smooth muscle cells. This novel study necessitates caution while interpreting the results from acetic acid bladder irritation model.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Pharmacology - Volume 563, Issues 1–3, 1 June 2007, Pages 203–208
نویسندگان
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