کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2539074 | 1122163 | 2010 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Proton translocating ATPase mediated fungicidal activity of eugenol and thymol Proton translocating ATPase mediated fungicidal activity of eugenol and thymol](/preview/png/2539074.png)
Eugenol (1) and thymol (2) exhibit excellent fungicidal activity against pathogenic yeasts, including isolates resistant to azoles. The rapid irreversible action of compound-1 and compound 2 on fungal cells suggested a membrane-located target for their action. We investigated their effect on H+-ATPase mediated H+-pumping by various Candida species. Both compounds inhibit H+-ATPase activity at their respective MIC values — 500 and 100 μg/ml. Glucose stimulated H+-extrusion was also inhibited significantly by compound 1 and compound 2. Inhibition of H+-ATPase leads to intracellular acidification and cell death. Inhibition of cell growth and H+-efflux by test compounds suggests that their antifungal properties are related to their inhibitory effects on H+-ATPase.
Effect of Eugenol(1) and Thymol(2) on H+-extrusion and glucose stimulated H+-extrusion by plasma membrane ATPase of Candida has been investigated and correlated with growth rate, viability and resistance.Figure optionsDownload as PowerPoint slide
Journal: Fitoterapia - Volume 81, Issue 8, December 2010, Pages 1157–1162