Supplementation of omega-3 fatty acids with aripiprazole and lithium lessens methylphenidate induced manic behavior in Swiss albino mice

• Methylphenidate (MPD) induced manic behavior in mice.
• The effect of supplementation of omega-3 fatty acid on MPD induced mania mice was examined by behavioral analysis such as open field test, forced swim test and actophotometer test.
• Supplementation of omega-3 fatty acids with lithium and aripiprazole shows utmost effect in decreasing manic behavior in MPD induced hyperlocomotion.

Bipolar disorder (BD) is a severe neuropsychiatric disorder, affecting approximately 1–2% of the world population. The present study designed to investigate whether omega-3 fatty acids could impinge the effect of methylphenidate (MPD) induced mania in mice after treatment with aripiprazole and lithium. Mice were subjected to mania paradigm daily for a period of 14 days to induce mania-like behavior by administration of MPD. The effect of supplementation of omega-3 fatty acids on MPD induced mania mice was examined by behavioral analysis such as open field test, forced swim test and actophotometer test. Supplementation of omega-3 fatty acids with aripiprazole and lithium reduced methylphenidate-induced hyperlocomotion. As a result MPD causes mania-like behavior in mice, as indicated by significant decrease in duration of immobility in forced swim test. Supplementation of omega-3 fatty acids (1.5 ml/kg), aripiprazole (1.5 mg/kg), lithium (50 mg/kg), significantly reduced the acute administration of MPD (5 mg/kg) induced mania-like behavior in open field test. Based on the behavioral analysis mice shows decreased total locomotion, duration of immobility time and the locomotor counts. In conclusion, the results of the present study suggested that omega-3 fatty acids supplementation alleviated the MPD-induced manic behavior in mice.

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