کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2572250 1561192 2015 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Short-term changes in intracellular ROS localisation after the silver nanoparticles exposure depending on particle size
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Short-term changes in intracellular ROS localisation after the silver nanoparticles exposure depending on particle size
چکیده انگلیسی


• Exposing cells to nanosilver particles (AgNPs) immediately induces ROS.
• Smaller AgNPs induce mitochondrial ROS production.
• AgNP-induced mitochondrial ROS are independent of particle internalisation.

Silver nanoparticles (AgNPs) induce the production of reactive oxygen species (ROS) and apoptosis. These effects are enhanced by smaller particles. Using live-cell imaging, we show that AgNPs induced ROS production rapidly in a size-dependent manner after exposure of cells to 70-nm and 1-nm AgNPs (AgNPs-70, AgNPs-1), but not AgNO3. Exposure of cells to 5 μg/mL each of AgNPs-70, AgNPs-1 or AgNO3 for 1 h decreased the cell viability by approximately 40%, 100% and 20%, respectively. ROS were rapidly induced after 5 and 60 min by AgNPs-1 and AgNPs-70, respectively, whereas AgNO3 had no detectable effect. ROS production detected using the reporter dichlorodihydrofluorescein was observed in whole cells and mitochondria 5 and 60 min after exposure to AgNPs-1. The present study is the first, to our knowledge, to report the temporal expression and intracellular localisation of ROS induced by AgNPs.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology Reports - Volume 2, 2015, Pages 574–579
نویسندگان
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