Alpha-ketoglutarate attenuates toxic effects of sodium nitroprusside and hydrogen peroxide in Drosophila melanogaster

• Dietary alpha-ketoglutarate partly abolishes toxic effects of sodium nitroprusside on D. melanogaster development.
• Dietary AKG prevents the increase in levels of oxidative stress markers in SNP-reared adult flies.
• Exogenous AKG effectively protects adult flies against H2O2 in combined treatments.
• AKG displays high H2O2-scavenging activity in vitro that explains its protective effects on flies under H2O2 treatment.

The protective effects of dietary alpha-ketoglutarate (AKG) are described that aid fruit flies, Drosophila melanogaster, to resist sodium nitroprusside (SNP) and hydrogen peroxide toxicity. Food supplementation with 10 mM AKG alleviated toxic effects of 1 mM SNP added to food and improved fly development. Dietary AKG also prevented the increase in levels of oxidative stress markers seen in SNP-reared adult flies. In vitro AKG did not affect the rate of SNP decomposition and did not bind iron and nitrite ions released in this process. Alpha-ketoglutarate also displayed high H2O2-scavenging activity in vitro and efficiently protected adult flies against this compound in combined treatments. Based on the observed antioxidant activity of AKG, it may be suggested that the antioxidant mode of AKG action (apart from its cyanide-binding capability) may be used to prevent the toxic effects of SNP and improve general physiological state of D. melanogaster and other animals and humans.

Figure optionsDownload as PowerPoint slide