کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2583294 1130685 2015 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Deoxynivalenol induces apoptosis in chicken splenic lymphocytes via the reactive oxygen species-mediated mitochondrial pathway
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Deoxynivalenol induces apoptosis in chicken splenic lymphocytes via the reactive oxygen species-mediated mitochondrial pathway
چکیده انگلیسی


• DON inhibited the growth of splenic lymphocytes in a dose-dependent manner.
• Cytotoxicity was assessed with the CCK-8 bioassay.
• DON treatment induced ROS accumulation in splenic lymphocytes.
• Apoptosis was correlated with inappropriate of p53, Bax, Bak-1, Bcl-2, and caspase-3 concentrations.
• Apoptosis was correlated with disordered of p53, Bax, Bak-1, Bcl-2, and caspase-3 expression.

We investigated the immunotoxicity and cytotoxicity of deoxynivalenol (DON), a mycotoxin, and the mechanism by which it induces apoptosis. Chicken splenic lymphocytes treated with 0–50 μg/mL DON for 48 h inhibited growth of splenic lymphocytes in a dose-dependent manner, as revealed by the Cell Counting Kit-8 (CCK-8) bioassay. Annexin V-fluorescein isothiocyanate staining indicated that the number of apoptotic and necrotic cells were significantly higher compared with the control (P < 0.01). DON treatment induced ROS accumulation, resulting in reduced mitochondrial transmembrane potential, as detected by flow cytometry and 2′,7′-dichlorofluorescein acetate and rhodamine 123 labeling, respectively. Enzyme linked immunosorbent assays revealed that the concentrations of p53, Bax, Bak-1, and Caspase-3 increased with increasing DON concentration (P < 0.05 or P < 0.01), whereas the concentrations of Bcl-2 decreased (P < 0.01) compared with the control. These data suggest that DON induces apoptosis in splenic lymphocytes via a ROS-mediated mitochondrial pathway.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Environmental Toxicology and Pharmacology - Volume 39, Issue 1, January 2015, Pages 339–346
نویسندگان
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