کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2583294 | 1130685 | 2015 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Deoxynivalenol induces apoptosis in chicken splenic lymphocytes via the reactive oxygen species-mediated mitochondrial pathway Deoxynivalenol induces apoptosis in chicken splenic lymphocytes via the reactive oxygen species-mediated mitochondrial pathway](/preview/png/2583294.png)
• DON inhibited the growth of splenic lymphocytes in a dose-dependent manner.
• Cytotoxicity was assessed with the CCK-8 bioassay.
• DON treatment induced ROS accumulation in splenic lymphocytes.
• Apoptosis was correlated with inappropriate of p53, Bax, Bak-1, Bcl-2, and caspase-3 concentrations.
• Apoptosis was correlated with disordered of p53, Bax, Bak-1, Bcl-2, and caspase-3 expression.
We investigated the immunotoxicity and cytotoxicity of deoxynivalenol (DON), a mycotoxin, and the mechanism by which it induces apoptosis. Chicken splenic lymphocytes treated with 0–50 μg/mL DON for 48 h inhibited growth of splenic lymphocytes in a dose-dependent manner, as revealed by the Cell Counting Kit-8 (CCK-8) bioassay. Annexin V-fluorescein isothiocyanate staining indicated that the number of apoptotic and necrotic cells were significantly higher compared with the control (P < 0.01). DON treatment induced ROS accumulation, resulting in reduced mitochondrial transmembrane potential, as detected by flow cytometry and 2′,7′-dichlorofluorescein acetate and rhodamine 123 labeling, respectively. Enzyme linked immunosorbent assays revealed that the concentrations of p53, Bax, Bak-1, and Caspase-3 increased with increasing DON concentration (P < 0.05 or P < 0.01), whereas the concentrations of Bcl-2 decreased (P < 0.01) compared with the control. These data suggest that DON induces apoptosis in splenic lymphocytes via a ROS-mediated mitochondrial pathway.
Journal: Environmental Toxicology and Pharmacology - Volume 39, Issue 1, January 2015, Pages 339–346