Alterations in activity and mRNA expression of acetylcholinesterase in the liver, kidney and gill of common carp exposed to atrazine and chlorpyrifos

Insecticides and herbicides are widely used in modern agricultural production. The intensive use of insecticide chlorpyrifos (CPF) and herbicide atrazine (ATR) has resulted in serious environmental problems. Herein, we investigated alteration in activity and mRNA levels of AChE in the liver, kidney and gill from common carp after 40 d exposure to CPF and ATR alone or in combination and 20 d recovery treatment. Results indicated that activity and mRNA levels of AChE at all high-dose groups have been significantly decreased after CPF and ATR alone or ATR/CPF mixture exposure, and the changes were improved in the end of recovery tests in varying degrees, the activity and gene expression of AChE in the joint toxicity of ATR and CPF groups were significantly lower than that in the single toxicant group. Our study suggests that the decrease of AChE activity observed at all high-dose groups (CPF and ATR alone or in combination) may be directly related to a lower AChE expression, and the joint toxicity of ATR and CPF is higher than ATR and CPF alone.

► Activity and transcription of AChE were investigated by ATR, CPF and their mixture.
► Intensive use of agricultural chemicals for a long time and their influence on aquatic ecosystems.
► Our aim was to evaluate the sub-chronic effects of ATR, CPF and their mixture on common carp.