کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2583461 1130691 2012 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification and expression profile of Id1 in bighead carp in response to microcystin-LR
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Identification and expression profile of Id1 in bighead carp in response to microcystin-LR
چکیده انگلیسی

Microcystin-LR (MCLR) is a widespread cyanotoxin produced in algal blooms, and has potent hepatotoxicity and tumor-promoting activity. We cloned the full-length cDNA of Id1 in bighead carp. The full-length Id1 cDNA was 954 bp and contained a 387 bp ORF. Bighead carp Id1 shared high identity with zebrafish Id1 amino acid sequence, and phylogenetic analysis showed that teleost Id1 evolved closely. Bighead carp Id1 constitutively expressed in all tested tissues in normal. When tested at two different time points post exposure and at 3 different MCLR doses, Id1 expression increased in a time-dependent pattern, and Id1 expression in brain was very sensitive to MCLR exposure. The present study will help us to understand more about the evolution of Id1 molecule and its role in the MCLR induced cell differentiation and cancer promoting in bighead carp.


► The full-length cDNA of Id1 in bighead carp was cloned.
► Bighead carp Id1 and other teleost Id1 evolved closely.
► Bighead carp Id1 expression increased in a time-dependent pattern in response to MCLR injection.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Environmental Toxicology and Pharmacology - Volume 34, Issue 2, September 2012, Pages 324–333
نویسندگان
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