Antioxidant and antigenotoxic effects of rosemary (Rosmarinus officinalis L.) extracts in Salmonella typhimurium TA98 and HepG2 cells

In the present study the chemopreventive effects of water soluble AquaROX® 15 and oil soluble VivOX® 40 rosemary extracts against 4-nitroquinoline-N-oxide (NQNO) and 2-amino-3-methyl-3H-imidazo[4,5-F]quinoline (IQ) induced mutagenicity in the reverse mutation assays with Salmonella typhimurium TA98 and against t-butyl hydroperoxide (t-BOOH), benzo(a)pyrene (BaP) and 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) induced DNA damage in HepG2 cells were studied, applying the comet assay. The results showed comparable protective effect of AquaROX and VivOX against oxidative DNA damage, whereas protection against indirect active genotoxic carcinogens was more efficient by VivOX.

• Rosmarinus officinalis extracts efficiently protected against bacterial mutagens, NQNO and IQ.
• The extracts protected human cells against ROS-inducing agent, t-BOOH.
• The extracts protected human cells against indirect mutagens, BaP and PhIP.
• The mechanisms of protection involve the modulation of cellular antioxidant responses and the effects on bioactivation and detoxification of xenobiotics.