Involvement of Sertoli cells in spermatogenic failure induced by carbendazim

The aim of this study is to determine whether Sertoli cells are involved in spermatogenic failure induced by carbendazim, a benzimidazole fungicide that is widely used in agriculture. Total 40 rats were treated with carbendazim by oral gavage at dosages of 0, 20, 100 and 200 mg/kg for 60 days. The testis weight, sperm counts, sperm motility and Sertoli cell morphology and secretions including vimentin, ABP, inhibin B, SCFs and SCFm in testis were examined. In 100 and 200 mg/kg groups, the testis weight, the sperm counts and motility and SCFm levels were significantly decreased, the sloughing of germ cells and ABP levels were increased, and the vimentin filaments and Sertoli cell morphology were altered. Inhibin B and SCFs levels were unchanged. The results suggested that alterations of Sertoli cell morphology and function were involved in spermatogenic failure induced by carbendazim.