کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2583979 | 1130710 | 2014 | 11 صفحه PDF | دانلود رایگان |
• Microarray analysis identified 15 miRNAs that were differentially expressed in OC-exposed A549 human alveolar cells.
• Integrated analyses of miRNA and mRNA expression profiles identified significant miRNA–mRNA anti-correlations.
• GO analysis of 101 putative target genes showed that the biological category ‘MAPK signaling pathway’ was prominently annotated.
• Phosphorylation of p38 MAPK was increased in OC-exposed A549 cells.
Previous environmental microRNA (miRNA) studies have investigated a limited number of candidate miRNAs and have not evaluated functional effects on gene expression. In this study, we aimed to identify octanal (OC)-sensitive miRNAs and to characterize the relationships between miRNAs and expression of candidate genes involved in OC-induced toxicity.Microarray analysis identified 15 miRNAs that were differentially expressed in OC-exposed A549 human alveolar cells. Integrated analyses of miRNA and mRNA expression profiles identified significant miRNA–mRNA anti-correlations. GO analysis of 101 putative target genes showed that the biological category ‘MAPK signaling pathway’ was prominently annotated. Moreover, we detected increased phosphorylation of p38 MAPK in the OC-exposed group.By integrating the transcriptome and microRNAome, we provide evidence that OC can affect MAPK-induced toxicity signaling. Therefore, this study demonstrates the added value of an integrated miRNA–mRNA approach for identifying molecular events induced by environmental pollutants in an in vitro human model
Journal: Environmental Toxicology and Pharmacology - Volume 37, Issue 1, January 2014, Pages 84–94