Impaired wound healing by exposure of different mainstream whole smoke solutions of commercial cigarettes

Cigarette smoke has been shown to potentiate wound damage and delayed ulcer healing. The chicken dorsum excisional wound assay was used to elucidate the deleterious effects of different mainstream whole smoke solutions (MSWSS) on the fundamental processes of wound healing. Gross, histopathology, SEM and computer based 3D image probing techniques were utilized to quantify different toxic effects of MSWSS on wound healing. A total of 160 chicks, aged 1 week, divided in eight groups were exposed to MSWSS with different nicotine concentration; 0.2 mg (group A), 0.3 mg (group B), 0.5 mg (group C), 0.6 mg (group D), 0.7 mg (group E) and 1 mg (group F). A very highly significant reduction (P < 0.001) in wound closure was observed among all MSWSS treated groups at day 8 post-wounding. Histological investigations revealed a significant impede outcome in the re-epithelialization of all MSWSS exposed wounds. Delayed dermal matrix regeneration and maturation of collagen bundles were observed among all MSWSS treated wounds. Similar results were achieved through SEM of treated wounds. Histological and image probing analysis unveiled the scanty neovascularization among MSWSS treated wounds. Abbot curve, angular spectrum and different other parameters of 3D surface topographies of wounds revealed a very highly significant reduction (P < 0.001) in angiogenesis among all MSWSS treated groups. These annotations validate the damaging effects of MSWSS on the healing of wounds.