Glycoprotein isolated from Ulmus davidiana Nakai modulates inflammatory related factors in mouse colonic tissues

This study was carried out to investigate the anti-inflammatory effects of a 116 kDa glycoprotein isolated from Ulmus davidiana Nakai (UDN glycoprotein) in dextran sodium sulfate (DSS)-treated mice. In DSS-treated mouse, the results showed that pretreatment with UDN glycoprotein [40 mg/kg body weight (BW)] normalized the augment of disease activity index (DAI) level and shortening of the large intestine. In addition, UDN glycoprotein inhibited myeloperoxidase (MPO) activation, thiobarbituric acid reactive substances (TBARS) formation, nitric oxide (NO) production and lactate dehydrogenase (LDH) release, whereas increased the activity of anti-oxidant enzymes [superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx)], accompanying the inhibition of pro-inflammatory-related signal mediators [colonic nuclear factor-kappa B (NF-κB), inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2)] in DSS-stimulated mice colitis. These results speculate that UDN glycoprotein may be useful for prevention of inflammatory disease and colorectal carcinogenesis.