کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2585552 1130875 2011 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Protective effect of N-acetyl-L-cysteine against maneb induced oxidative and apoptotic injury in Chinese hamster V79 cells
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
Protective effect of N-acetyl-L-cysteine against maneb induced oxidative and apoptotic injury in Chinese hamster V79 cells
چکیده انگلیسی

The role of antioxidant N-acetyl-L-cysteine (NAC) in protection against cellular changes triggered by maneb during in vitro exposure was investigated in cultured Chinese hamster V79 cells. We observed high apoptotic activity and high oxidative stress induced by exposure to maneb evidenced by a statistically significant increase in lipid peroxidation (measured as TBARS – thiobarbituric acid reactive substances) as well as a decrease of glutathione (GSH) and glutathione disulfide (GSSG) ratio (GSH/GSSG). Maneb did not exhibit any effect on protein oxidation (measured by protein carbonyls content). NAC suppressed cellular changes induced by maneb in V79 cells. NAC pre-treatment prevented TBARS production and significantly decreased the number of apoptotic cells. However, protective effect of NAC on GSH and GSSG levels has been shown only in cells exposed to lower concentration of maneb (100 μM).

Research highlights
► Prooxidative and proapoptotic properties of maneb was investigated in vitro in Chinese hamster V79 cells.
► The role of antioxidant NAC in protection against cellular effects triggered by maneb was revealed.
► NAC pre-treatment of cells prevented lipid peroxidation, decreased the number of apoptotic cells as well as suppressed changes in glutathione homeostasis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food and Chemical Toxicology - Volume 49, Issue 4, April 2011, Pages 1020–1025
نویسندگان
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