کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2586834 1130911 2009 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Modulation of human neutrophil oxidative metabolism and degranulation by extract of Tamarindus indica L. fruit pulp
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
Modulation of human neutrophil oxidative metabolism and degranulation by extract of Tamarindus indica L. fruit pulp
چکیده انگلیسی

The tamarind (Tamarindus indica L.) is indigenous to Asian countries and widely cultivated in the American continents. The tamarind fruit pulp extract (ExT), traditionally used in spices, food components and juices, is rich in polyphenols that have demonstrated anti-atherosclerotic, antioxidant and immunomodulatory activities. This study evaluated the modulator effect of a crude hydroalcoholic ExT on some peripheral human neutrophil functions. The neutrophil reactive oxygen species generation, triggered by opsonized zymosan (OZ), n-formyl-methionyl-leucyl-phenylalanine (fMLP) or phorbol myristate acetate (PMA), and assessed by luminol- and lucigenin-enhanced chemiluminescence (LumCL and LucCL, respectively), was inhibited by ExT in a concentration-dependent manner. ExT was a more effective inhibitor of the PMA-stimulated neutrophil function [IC50 (in μg/106cells) = 115.7 ± 9.7 (LumCL) and 174.5 ± 25.9 (LucCL)], than the OZ- [IC50 = 248.5 ± 23.1 (LumCL) and 324.1 ± 34.6 (LucCL)] or fMLP-stimulated cells [IC50 = 178.5 ± 12.2 (LumCL)]. The ExT also inhibited neutrophil NADPH oxidase activity (evaluated by O2 consumption), degranulation and elastase activity (evaluated by spectrophotometric methods) at concentrations higher than 200 μg/106 cells, without being toxic to the cells, under the conditions assessed. Together, these results indicate the potential of ExT as a source of compounds that can modulate the neutrophil-mediated inflammatory diseases.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food and Chemical Toxicology - Volume 47, Issue 1, January 2009, Pages 163–170
نویسندگان
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