کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2587125 | 1130917 | 2008 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effect of diisopropanolamine upon choline uptake and phospholipid synthesis in Chinese hamster ovary cells
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کلمات کلیدی
PBSNTPDEATLCDIPAsphingomyelin - اسفنگومیلینCho - برایNational Toxicology Program - برنامه سم شناسی ملیChinese Hamster Ovary - تخمدان هامستر چینیdiethanolamine - دی اتانولینDiisopropanolamine - دی ایزوپروپانولامینPhospholipid synthesis - سنتز فسفولیپیدPhosphate buffered saline - فسفات بافر شورthin-layer-chromatography - کروماتوگرافی نازک لایهCholine - کولین
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Aminoalcohols differ in mammalian toxicity at least in part based upon their ability to alter the metabolism of phospholipids and to cause depletion of the essential nutrient choline in animals. This study examined the incorporation of diisopropanolamine (DIPA) into phospholipids (PLs) and effects of DIPA upon choline uptake and phospholipid synthesis in Chinese hamster ovary (CHO) cells. Results were compared to those of a related secondary alcohol amine, diethanolamine (DEA), whose systemic toxicity is closely associated with its metabolic incorporation into PLs and depletion of choline pools. DIPA caused a dose-related inhibition of 3H-choline uptake by CHO cells that was approximately 3-4 fold less potent, based upon an IC50, than that reported for DEA. DIPA, in contrast to DEA, did not cause changes in the synthesis rates of 33P-phosphatidylethanolamine, 33P-phosphatidylcholine or 33P-sphingomyelin at either non-toxic or moderately toxic concentrations. Only approximately 0.004%, of administered 14C-DIPA was metabolically incorporated into PLs, over 30-fold less than the incorporation of 14C-DEA under similar conditions. Overall, these data and previous pharmacokinetic and toxicity data obtained in vivo suggests that DIPA is distinct from DEA and lacks significant choline and PL metabolism related toxicity in animals.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food and Chemical Toxicology - Volume 46, Issue 2, February 2008, Pages 761-766
Journal: Food and Chemical Toxicology - Volume 46, Issue 2, February 2008, Pages 761-766
نویسندگان
W.T. Stott, K.M. Kleinert,