کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2596682 | 1132542 | 2009 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Tri-n-butyltin increases intracellular Zn2+ concentration by decreasing cellular thiol content in rat thymocytes
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
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چکیده انگلیسی
Effect of tri-n-butyltin (TBT), an environmental pollutant, on intracellular Zn2+ concentration was tested in rat thymocytes to reveal one of cytotoxic profiles of TBT at nanomolar concentrations using a flow cytometer and appropriate fluorescent probes. TBT at concentrations of 30Â nM or more (up to 300Â nM) significantly increased the intensity of FluoZin-3 fluorescence, an indicator for intracellular Zn2+ concentration, under external Ca2+- and Zn2+-free condition. Chelating intracellular Zn2+ completely attenuated the TBT-induced augmentation of FluoZin-3 fluorescence. Result suggests that nanomolar TBT releases Zn2+ from intracellular store site. Oxidative stress induced by hydrogen peroxide also increased the FluoZin-3 fluorescence intensity. The effects of TBT and hydrogen peroxide on the fluorescence were additive. TBT-induced changes in the fluorescence of FluoZin-3 and 5-chloromethylfluorescein, an indicator for cellular thiol content, were correlated with a coefficient of â0.962. Result suggests that the intracellular Zn2+ release by TBT is associated with TBT-induced reduction of cellular thiol content. However, chelating intracellular Zn2+ potentiated the cytotoxicity of TBT. Therefore, the TBT-induced increase in intracellular Zn2+ concentration may be a type of stress responses to protect the cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology - Volume 262, Issue 3, 21 August 2009, Pages 245-249
Journal: Toxicology - Volume 262, Issue 3, 21 August 2009, Pages 245-249
نویسندگان
Toshihisa B. Oyama, Keisuke Oyama, Takuya Kawanai, Tomohiro M. Oyama, Erika Hashimoto, Masaya Satoh, Yasuo Oyama,