کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2598239 1562437 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterization of nigerlysin ©, hemolysin produced by Aspergillus niger, and effect on mouse neuronal cells in vitro
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Characterization of nigerlysin ©, hemolysin produced by Aspergillus niger, and effect on mouse neuronal cells in vitro
چکیده انگلیسی

Aspergillus niger produced a proteinaceous hemolysin, nigerlysin © when incubated on sheep's blood agar (SBA) at both 23 and 37 °C. Nigerlysin was purified from tryptic soy broth (TSB) culture filtrate and found to have a molecular weight of approximately 72 kDa, with an isoelectric point of 3.45. Nigerlysin is heat stable up to 65 °C but unstable at 75 °C when incubated for 10 min. Circular dichroic analysis revealed that nigerlysin has an alpha helical structure. Exposure of mouse primary cortical neuronal cells to 0.1 μg ml−1 of nigerlysin resulted in the rapid loss of their viability, approximately 50% in 24 h. The IC50 is estimated to be 0.037 μg ml−1, or between 0.034 and 0.041 μg ml−1 at the 95% confidence level.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology - Volume 219, Issues 1–3, 15 February 2006, Pages 150–155
نویسندگان
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