کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2598239 | 1562437 | 2006 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization of nigerlysin ©, hemolysin produced by Aspergillus niger, and effect on mouse neuronal cells in vitro
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
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چکیده انگلیسی
Aspergillus niger produced a proteinaceous hemolysin, nigerlysin © when incubated on sheep's blood agar (SBA) at both 23 and 37 °C. Nigerlysin was purified from tryptic soy broth (TSB) culture filtrate and found to have a molecular weight of approximately 72 kDa, with an isoelectric point of 3.45. Nigerlysin is heat stable up to 65 °C but unstable at 75 °C when incubated for 10 min. Circular dichroic analysis revealed that nigerlysin has an alpha helical structure. Exposure of mouse primary cortical neuronal cells to 0.1 μg ml−1 of nigerlysin resulted in the rapid loss of their viability, approximately 50% in 24 h. The IC50 is estimated to be 0.037 μg ml−1, or between 0.034 and 0.041 μg ml−1 at the 95% confidence level.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology - Volume 219, Issues 1–3, 15 February 2006, Pages 150–155
Journal: Toxicology - Volume 219, Issues 1–3, 15 February 2006, Pages 150–155
نویسندگان
Maura Donohue, Wei Wei, Jinfang Wu, Nasser H. Zawia, Nicholas Hud, Victor De Jesus, Detlef Schmechel, Justin M. Hettick, Donald H. Beezhold, Stephen Vesper,