کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2684433 | 1142747 | 2007 | 5 صفحه PDF | دانلود رایگان |
SummaryBackground & aimsPrevious studies of our research group have shown that apoptosis is present in skeletal muscle of tumour-bearing animals subject to cachexia. For this reason we decided to investigate the apoptosis in skeletal muscle of cancer patients.Methods and resultsIn the present study, muscle biopsies from weight-losing patients with upper gastro-intestinal cancer showed a significant increase in muscle DNA fragmentation (three-fold), as compared with control subjects. The increase in DNA laddering was associated with an increase in poly(ADP-ribose) polymerase (PARP) cleavage (four-fold) as measured by western blotting. These two events indicate the presence of muscle apoptosis. These changes were associated with a decrease in MyoD protein content, suggesting important alterations in skeletal muscle physiology.ConclusionsThe results presented therefore confirm that apoptosis is also present in human subjects undergoing cancer cachexia.
Journal: Clinical Nutrition - Volume 26, Issue 5, October 2007, Pages 614–618