کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2684549 | 1142756 | 2006 | 12 صفحه PDF | دانلود رایگان |
SummaryBackground and aimsThere is increased interest in the study of manipulation of the flora with pro- and prebiotics regarding inflammatory bowel disease. The aim of this work was to evaluate the effect of oligosaccharides from goat milk in a rat model of dextran sodium sulfate- (DSS-) induced colitis.MethodsTwenty rats were fed the same diet but with different sources of fiber (5% of the diet): cellulose or a mixture of goat's milk oligosaccharides (GMO) and cellulose. DSS treatment was used to induce a colonic inflammation. Several clinical and inflammatory parameters, as well as intestinal micorbiota and gene expression by DNA microarray technology, were evaluated.ResultsDSS induced a decrease in body weight which was not observed in rats fed the GMO (decrease of 21±11% in control rats vs increase of 5.2±8.6 in GMO rats, P<0.05P<0.05). DSS also caused an acute colonic inflammatory process which was weaker in rats fed the GMO, as shown by colon myeloperoxidase activity (0.53±0.16 vs 0.14±0.07 U/mg of protein, P<0.05P<0.05), as well as clinical symptoms measured by a scoring system (1.25±1.14 vs 0.4±0.07, P<0.05P<0.05). GMO rats also showed less severe colonic lesions and a more favorable intestinal microbiota. The expression of genes involved in intestinal function, such as mucine-3, was down-regulated in DSS-control rats but returned to normal values in GMO rats.ConclusionGMO reduce intestinal inflammation and contribute to the recovery of damaged colonic mucosa.
Journal: Clinical Nutrition - Volume 25, Issue 3, June 2006, Pages 477–488