کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2689747 1143221 2013 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Novel multi-sugar assay for site-specific gastrointestinal permeability analysis: A randomized controlled crossover trial
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی مراقبت های ویژه و مراقبتهای ویژه پزشکی
پیش نمایش صفحه اول مقاله
Novel multi-sugar assay for site-specific gastrointestinal permeability analysis: A randomized controlled crossover trial
چکیده انگلیسی

SummaryBackground & aimsIncreased gastrointestinal (GI) permeability is an important hallmark of many conditions, potentially leading to antigen exposure and sepsis. Current permeability tests are hampered by analytical limitations. This study aims to compare the accuracy of our multi-sugar (MS) and the classical dual sugar (DS) test for detection of increased GI permeability.MethodsTen volunteers received permeability analysis using MS (1 g sucrose, lactulose, sucralose, erythritol, 0.5 g rhamnose in water) or DS (5 g lactulose, 0.5 g rhamnose), after indomethacin or placebo. Blood and urine were analyzed by isocratic LC-MS.ResultsMS testing revealed significantly elevated urinary lactulose/rhamnose (L/R) ratios after indomethacin, due to enhanced lactulose excretion (P < .01) and unaltered rhamnose excretion. The DS test showed increased L/R ratios, due to increased lactulose excretion and decreased rhamnose excretion (both P < .05). After indomethacin, plasma L/R increased in both assays (P < .05 and P < .01). Urinary and plasma L/R ratios correlated significantly. Indomethacin increased sucrose excretion and 0–1 h sucrose/rhamnose. Colon permeability was unchanged.ConclusionsSensitive permeability analysis is feasible in plasma and urine using MS or DS test. In contrast to the DS test, monosaccharide excretion is not decreased by the MS test. In short, the MS test provides accurate, site-specific information on gastroduodenal, small, and large intestinal permeability.Registered at US National Library of Medicine (http://www.clinicaltrials.gov, NCT00943345).

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Clinical Nutrition - Volume 32, Issue 2, April 2013, Pages 245–251
نویسندگان
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