Lens-care-solution-induced alterations in dynamic interfacial properties of human tear-lipid films

• Tear lipids were collected using Schirmer strips, lipids were extracted and deposited as thick films at air–water interface.
• Dynamic interfacial and rheological behavior were evaluated for these lipid films alone and with LCS added to aqueous phase.
• ClearCare and Revitalens produced less significant changes in lipid films dynamic properties than other LCS studied.

PurposeTo evaluate the influence of lens care solutions (LCS) on interfacial dynamics and rheological properties of human tear-lipid films.MethodsTear lipids were extracted from Schirmer strips collected from 6 healthy subjects. Sessile bubble tensiometry was used to study interfacial properties at 22 °C. Lipids were deposited on an air bubble immersed into electrolytes solution to form 90 ± 20 nm films. Lipid films were subjected to expansion-compression cycles for dynamic interfacial properties and to step-strain relaxations for assessments of rheological properties. LCS (BioTrue [BT], PureMoist [PM], Revitalens [RL], ClearCare [CC]) were injected into optical chamber and equilibrated for 2 h without or with lipid films. Dynamic interfacial properties of films were measured. Then electrolyte solution was pumped through chamber and properties of films were re-evaluated.ResultsEquilibrium surface tension (EST), elasticity modulus (E), and relaxation times (τ) of tear lipids were 22 ± 2.1 mN/m, 10.7–14.8 mN/m, and 80–150 s, respectively. EST for LCS was 45.3 ± 0.8 for CC, 40.3 ± 0.8 for BT, 33.4 ± 1.0 for PM, and 30.1 ± 0.8 mN/m for RL. E for LCS varied within 0.5–6.7 mN/m, and τ varied from 49 to 68 ± 5 s. For mixed lipids + LCS films, EST remained unchanged whereas E and τ were reduced for all LCS types. Exposure to PM and RL noticeably altered the shape of lipid-film iso-cycles. These changes persisted after LCS washout.ConclusionsSome components of LCS bind irreversibly to lipid films and make them less viscous and less elastic. These findings suggest the possibility of tear-film destabilization upon LCS exposure.