کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2699297 | 1144164 | 2012 | 8 صفحه PDF | دانلود رایگان |
PurposeTo explore the in vitro effects of multipurpose contact lens solutions (MPSs) on corneal epithelial barrier function and viability.MethodsHuman corneal epithelial cells (HCEpiC) were exposed to 50% MPSs A–G. Viability was determined using metabolic activity, protease release and caspase assays. Barrier function was evaluated using immunostaining for the tight junction protein zonnula occludens-1 (ZO-1) and resistance measurements.ResultsMPS A and G did not affect HCEpiC monolayer viability after 2 h, while MPSs B–F significantly decreased viability. There was a significant decrease in stratified HCEpiC viability after exposure to MPSs B–E for 2 h, while there was no effect of MPS A. After exposure of HCEpiC monolayers to MPS A, F or G for 30 min, ZO-1 staining appeared similar to control. HCEpiC exposed to MPSs B and C demonstrated tight junction breakdown. There was no significant change in HCEpiC monolayer resistance after exposure to MPS A or F for 2 h, while MPSs B–E and G reduced resistance. After exposure to MPS A–E, stratified HCEpiC resistance was significantly decreased after 2 or 4 h. The decrease in resistance was significantly less with MPS A as compared to the other MPSs.ConclusionsMPSs caused varying modifications to cell viability and barrier function in monolayer and stratified HCEpiC. MPS A did not alter monolayer HCEpiC viability or barrier function, while MPSs B–G caused significant decreases of at least one parameter. Furthermore, MPS A had significantly less effect than MPSs B–E on viability and barrier function of stratified HCEpiC.
Journal: Contact Lens and Anterior Eye - Volume 35, Issue 4, August 2012, Pages 163–170