کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2775957 | 1152354 | 2017 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Comparison of cell interactions with laser machined micron- and nanoscale features in polymer
ترجمه فارسی عنوان
مقایسه تعاملات سلولی با ویژگی های میکرون و نانومواد لیزر در پلیمر
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کلمات کلیدی
میکروسکوپ الکترونی اسکن، برچسب ایمنولوژیک، نانوساختار،
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوشیمی بالینی
چکیده انگلیسی
Control of cell responses to artificial surfaces is a research goal for much of the biomaterials community. The role that the micron scale topography of a surface can play in controlling cell responses has been well documented and recent advances in nanofabrication techniques have lead to an interest in cells' responses to submicron-scale surface features. The study described here compares the relative influences that nanoscale and micron-scale features exert on cells by examining cytoskeletal organisation. Micron-scale structures were generated on the polyamide Kapton® using a 193Â nm ArF Excimer laser, at 400Â mJ/cm2 fluence. Nanoscale features were generated on Kapton using the excimer laser with a phase mask. Osteoblasts were seeded onto surfaces for 24Â h, then the cell membranes were detergent-extracted, and the cells were applied with a primary antibody to actin and a colloidal gold-conjugated secondary antibody. Samples to be examined using the confocal were mounted in glycerol, those for electron microscopy were carbon-coated. The organisation of actin was examined on micron- and nano-scale structures by scoring sections for order of branching and angles of branching to relate changes in the cytoskeleton relative to the control. Although there was a strong influence of micron-scale structures, the cytoskeleton of cells on the nanoscale structures were similar to the controls.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental and Molecular Pathology - Brought to you by:College of Engineering Chengannur - 'Renewal due by 31 Dec 2017'
Journal: Experimental and Molecular Pathology - Brought to you by:College of Engineering Chengannur - 'Renewal due by 31 Dec 2017'
نویسندگان
Michael D. Ball, Una Prendergast, Claire O'Connell, Richard Sherlock,