کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2774830 1152296 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Hyperoxia disrupts the intestinal barrier in newborn rats
ترجمه فارسی عنوان
هیپرکسیا موانع روده ای را در موش های نوزاد تازه متولد شده مختل می کند
کلمات کلیدی
IL، اینترلوکین؛ I-FABP، پروتئین مرتبط با اسید چرب روده؛ 8-OHdG، 8-هیدروکسی-2'-deoxyguanosine؛ PBS، نمک فسفات بافر؛ RA، هوای اتاق. ZO، zonula occludensHyperoxia؛ پروتئین مرتبط با اسید چرب روده؛ Occludin؛ occludens Zonula
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی بالینی
چکیده انگلیسی

Animal studies have demonstrated that neonatal hyperoxia injures the distal small intestine. This study aimed to determine the effects of neonatal hyperoxia exposure on the intestinal morphology and intestinal barrier integrity in newborn rats. Sprague–Dawley rat pups were exposed to either ambient air or hyperoxia. The ambient air and normobaric hyperoxia groups were maintained in room air and 85% O2 for 2 weeks, respectively. The rats were euthanized on Postnatal Day 14, and the terminal ileum was collected for histological analyses and oxidative stress measurements. The generation of reactive oxygen species was evaluated by measuring the production of 8-hydroxy-2′-deoxyguanosine (8-OHdG). The expression and localization of epithelial injury markers [intestinal fatty acid binding protein (I-FABP)] and intestinal barrier proteins [occludin and zonula occludens (ZO)-1] were analyzed through immunofluorescence staining and western blotting. The body weight at birth was comparable between the two groups. On Postnatal Day 14, the rats in the hyperoxia group exhibited significantly lower body weight, a higher serum interleukin-6 level, a higher intestinal injury score, higher 8-OHdG and I-FABP expression, and lower occludin and ZO-1 protein expression than did those in the ambient air group. Hyperoxia exposure injured the distal small intestine and disrupted the intestinal barrier in newborn rats. This may be attributable to oxidative stress during the postnatal period.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental and Molecular Pathology - Volume 101, Issue 1, August 2016, Pages 44–49
نویسندگان
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