کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2776798 1152636 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
In vitro and in vivo imaging of intracellular Ca2+ responses in salivary gland cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی بالینی
پیش نمایش صفحه اول مقاله
In vitro and in vivo imaging of intracellular Ca2+ responses in salivary gland cells
چکیده انگلیسی

BackgroundSalivary glands are comprised predominantly of two epithelial cell types, acinar and ductal cells. Activation of muscarinic acetylcholine receptors on acinar cells induces inositol 1,4,5-trisphosphate-mediated Ca2+ release from intracellular Ca2+ stores, followed by Ca2+ influx across the plasma membrane. The increase in intracellular Ca2+ concentration ([Ca2+]i) activates ion channels and transporters involved in fluid secretion; thus [Ca2+]i acts as the primary fluid secretion signal in acinar cells.HighlightThe development of Ca2+ imaging techniques has enabled extensive study of spatiotemporal Ca2+ dynamics in isolated acinar cells; however, the relationship between Ca2+ responses and fluid secretion in vivo is not yet fully understood. In vivo Ca2+ imaging in salivary gland cells is necessary for determining Ca2+ responses during salivary secretion. Recently, we developed an intravital imaging system for monitoring Ca2+ responses in rat submandibular gland (SMG) acinar cells in live animals using retrograde ductal injection of adenoviral vectors expressing the genetically encoded Ca2+ indicator, YC-Nano50. Expression of YC-Nano50 was observed throughout the SMG, and we confirmed that YC-Nano50 was functional for monitoring Ca2+ responses in SMG cells. Administration of acetylcholine significantly increased [Ca2+]i in the SMG, and this increase gradually decreased to resting levels.ConclusionWe successfully visualized agonist-induced Ca2+ dynamics in SMG acinar cells in live animals. This novel experimental system represents a powerful tool for understanding the relationships between Ca2+ responses and salivary secretion in vivo.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Oral Biosciences - Volume 57, Issue 2, May 2015, Pages 69–75
نویسندگان
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