کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2785591 1568380 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Intracerebroventricular d-galactose administration impairs memory and alters activity and expression of acetylcholinesterase in the rat
ترجمه فارسی عنوان
تجویز d-گالاکتوز Intracerebroventricular حافظه را کاهش می دهد و فعالیت و بیان استیل کولین استراز را در موش صحرایی تغییر می دهد
کلمات کلیدی
گالاکتوزمی کلاسیک؛ حافظه؛ فعالیت آنزیم استیل کولین استراز؛ بیان ژن
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی تکاملی
چکیده انگلیسی


• Galactose alters AChE activity and expression in the hippocampus of rats.
• Galactose impairs aversive memory in rats.
• Galactose impairs object recognition memory in rats.

Tissue accumulation of galactose is a hallmark in classical galactosemia. Cognitive deficit is a symptom of this disease which is poorly understood. The aim of this study was to investigate the effects of intracerebroventricular administration of galactose on memory (inhibitory avoidance and novel object recognition tasks) of adult rats. We also investigated the effects of galactose on acetylcholinesterase (AChE) activity, immunocontent and gene expression in hippocampus and cerebral cortex. Wistar rats received a single injection of galactose (4 mM) or saline (control). For behavioral parameters, galactose was injected 1 h or 24 h previously to the testing. For biochemical assessment, animals were decapitated 1 h, 3 h or 24 h after galactose or saline injection; hippocampus and cerebral cortex were dissected. Results showed that galactose impairs the memory formation process in aversive memory (inhibitory avoidance task) and recognition memory (novel object recognition task) in rats. The activity of AChE was increased, whereas the gene expression of this enzyme was decreased in hippocampus, but not in cerebral cortex. These findings suggest that these changes in AChE may, at least in part, to lead to memory impairment caused by galactose. Taken together, our results can help understand the etiopathology of classical galactosemia.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Developmental Neuroscience - Volume 50, May 2016, Pages 1–6
نویسندگان
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