Spatial relationship between NSCs/NPCs and microvessels in rat brain along prenatal and postnatal development

• The number of NSCs/NPCs around microvessels was quantified during pre- and postnatal brain development.
• Neurogenesis and angiogenesis were in close proximity to each other during prenatal and postnatal development.
• The spatial relationship between NSCs/NPCs and microvessels in neurogenic brain regions differed from non-neurogenic brain regions.
• Neurogenesis and angiogenesis during development have a different spatial–temporal sequence.
• NSCs/NPCs might produce some signals to regulate vasculature modeling.

Neurogenesis and angiogenesis are two parallel processes that occur in brain development and repair, and so share some molecular signals. In order to better understand the interaction between the genesis of neural cells and vessels during brain development, the density of microvessels and the number of nestin positive neural stem/neural progenitor cells (NSCs/NPCs) around microvasculature in various brain regions was quantified. Results showed that the density of microvessels remained at a relative low level during embryonic development and dramatically increased after postnatal day 3 (P3), especially in subventricular zone. The number of nestin positive NSCs/NPCs per microvessel in neurogenic brain regions continually increased with fetal brain development and then gradually dropped down during postnatal development. The highest density of NSCs/NPCs appeared at postnatal day 1 (P1) and dramatically decreased after P3. Similar pattern was observed in striatum. In the olfactory bulb, the cerebral cortex and cerebellum, the dramatic decrease of NSCs/NPCs density appeared after P7, especially in the cerebral cortex. Our results demonstrated that anatomically, the spatial relationship between NSCs/NPCs and microvessels changed during brain development. The alteration patterns in neurogenic brain regions differed from non-neurogenic brain regions.