کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2786219 1568410 2012 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The Brown Norway opticospinal model of demyelination: Does it mimic multiple sclerosis or neuromyelitis optica?
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی تکاملی
پیش نمایش صفحه اول مقاله
The Brown Norway opticospinal model of demyelination: Does it mimic multiple sclerosis or neuromyelitis optica?
چکیده انگلیسی

Opticospinal demyelinating diseases in humans are mostly characterized by the opticospinal form of multiple sclerosis (MS) and neuromyelitis optica (NMO). Increasing attention has recently focused on astrocyte markers, aquaporin-4 (AQP4) and glial fibrillary acidic protein (GFAP) in these diseases. We induced opticospinal demyelination in Brown Norway rats with soluble recombinant rat myelin oligodendrocyte glycoprotein (1–116) and incomplete Freund's adjuvant. Clinical, MRI, neuropathological and immunological evaluations were performed, with a focus on AQP4 and GFAP. We confirmed the opticospinal phenotype, including extensive myelitis, but also showed the MRI-characterized involvement of the periventricular area. Expression levels of myelin, AQP4 and GFAP showed the early involvement of astrocytes before demyelination in the optic nerve. The overexpression of AQP4 was particularly pronounced in the spinal cord and was concomitant with demyelination and astrocyte apoptosis. The disability scores were correlated with demyelination and inflammation but not with AQP4/GFAP expression. No antibodies against the linear and conformational epitopes of AQP4 were detected. Whereas a NMO-like phenotype was observed in this model, the AQP4/GFAP expression during the disease process was more closely related to opticospinal MS than NMO. However, this model raises the question of a continuum between opticospinal MS and the seronegative NMO subtype.


► Periventricular area is involved in the Brown Norway MOG-induced opticospinal model of demyelination.
► Markers of astrocyte expression (AQP4 and GFAP) are significantly modified in opticospinal region and periventricular area.
► Disability scores were correlated with demyelination and inflammation but not with AQP4/GFAP expression.
► A neuromyelitis optica-phenotype was observed in this model but without any antibodies against AQP4.
► This model has characteristics of both opticospinal multiple sclerosis and neuromyelitis optica.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Developmental Neuroscience - Volume 30, Issue 6, October 2012, Pages 487–497
نویسندگان
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