کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2786269 1568412 2012 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Expression of coat proteins changes during postnatal development in selected areas of the rat brain
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی تکاملی
پیش نمایش صفحه اول مقاله
Expression of coat proteins changes during postnatal development in selected areas of the rat brain
چکیده انگلیسی

It is well known that clathrin-mediated endocytosis is crucial for the normal functioning and integrity of neurons in the central nervous system. In this study we attempted to correlate the expression of coat proteins with development in different areas of rat brain. By Western blot, we studied the expression of AP-2, GGA1 and GGA2 in striatum, cerebellum, brain stem, cerebral cortex and hippocampus of newborn rats and during post-natal development; 5, 15, 30, 60, 90 or 150 days after birth. We observed that the expression of the α2 subunit of AP-2 increased substantially between the 15th and 30th day after birth in all areas studied, excepting the cerebellum and cortex. On the other hand, the expression of the α1 subunit does not change significantly during the development in any of the areas under study. We also noted that the expression of the μ2 subunit did not follow the pattern of α2 during development. In general terms, the expression of GGA1 and GGA2 followed a similar pattern to that of AP-2, although these proteins increased significantly in the cerebral cortex from the 15th day after birth. Moreover, presenilin-1, a protein associated with aging and neurodegeneration, shows an expression pattern similar to coat proteins in the striatum and cortex. These results suggest that proteins that conform the intracellular transport machinery in the brain cells seems to accompany development, according to the maturation of the different brain areas.


► We examined the expression of coat proteins in selected areas of rat brain during postnatal development.
► Expression of AP-2, GGA1 and GGA2 tend to increase between 15 and 30 days of age in all areas studied.
► The transport machinery could be related to the strong growth of nerve terminals.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Developmental Neuroscience - Volume 30, Issue 4, June 2012, Pages 333–341
نویسندگان
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