کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2786452 | 1568417 | 2011 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Dietary phytochemicals induce p53- and caspase-independent cell death in human neuroblastoma cells Dietary phytochemicals induce p53- and caspase-independent cell death in human neuroblastoma cells](/preview/png/2786452.png)
Neuroblastoma (NB) is the most prevalent pediatric solid tumor and a leading cause of cancer-related death in children. In the present study, a novel cytotoxic role for the dietary compounds, curcumin, andrographolide, wedelolactone, dibenzoylmethane, and tanshinone IIA was identified in human S-type NB cells, SK-N-AS and SK-N-BE(2). Mechanistically, cell death appeared apoptotic by flow cytometry; however, these effects proceeded independently from both caspase-3 and p53 activation, as assessed by both genetic (shRNA) and pharmacological approaches. Notably, cell death induced by both curcumin and andrographolide was associated with decreased NFκB activity and a reduction in Bcl-2 and Bcl-xL expression. Finally, curcumin and andrographolide increased cytotoxicity following co-treatment with either cisplatin or doxorubicin, two chemotherapeutic agents widely used in the clinical management of NB. Coupled with the documented safety in humans, dietary compounds may represent a potential adjunct therapy for NB.
• A novel effect of dietary compounds on neuroblastoma viability is presented.
• A p53- and caspase-3 independent mechanism of cell death is demonstrated.
• Cell death is associated with reduced activation of NFκB transcription factor.
• Dietary compounds potentiate the cytotoxicity of chemotherapeutic drugs.
Journal: International Journal of Developmental Neuroscience - Volume 29, Issue 7, November 2011, Pages 701–710