کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2786735 | 1568425 | 2010 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: CD133+ cells from human umbilical cord blood reduce cortical damage and promote axonal growth in neonatal rat organ co-cultures exposed to hypoxia CD133+ cells from human umbilical cord blood reduce cortical damage and promote axonal growth in neonatal rat organ co-cultures exposed to hypoxia](/preview/png/2786735.png)
To evaluate the effect of CD133+ cells (endothelial progenitor cells) on the hypoxia-induced suppression of axonal growth of cortical neurons and the destruction of blood vessels (endothelial cells), we used anterograde axonal tracing and immunofluorescence in organ co-cultures of the cortex and the spinal cord from 3-day-old neonatal rats. CD133+ cells prepared from human umbilical cord blood were added to the organ co-cultures after hypoxic insult, and axonal growth, vascular damage and apoptosis were evaluated. Anterograde axonal tracing with 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate was used to analyze axonal projections from the cortex to the spinal cord. Immunolabeling co-cultured tissues of the cortex and the spinal cord were used to investigate the effect of CD133+ cells on the survival of blood vessels and apoptosis in the brain cortex. Hypoxia remarkably suppressed axonal growth in organ co-cultures of the cortex and the spinal cord, and this suppression was significantly restored by the addition of CD133+ cells. CD133+ cells also reduced the hypoxia-induced destruction of the cortical blood vessels and apoptosis. CD133+ cells had protective effects on hypoxia-induced injury of neurons and blood vessels of the brain cortex in vitro. These results suggest that CD133+ cell transplantation may be a possible therapeutic intervention for perinatal hypoxia-induced brain injury.
Research highlights▶ CD133+ cells restore the hypoxia-induced suppression of axonal growth. ▶ CD133+ cells reduce the hypoxia-induced destruction of the cortical blood vessels. ▶ CD133+ cells reduce the hypoxia-induced apoptosis in the brain cortex.
Journal: International Journal of Developmental Neuroscience - Volume 28, Issue 7, November 2010, Pages 581–587