کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2789004 | 1154464 | 2012 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Galectin fingerprinting detects differences in expression profiles between bovine endometrium and placentomes as well as early and late gestational stages Galectin fingerprinting detects differences in expression profiles between bovine endometrium and placentomes as well as early and late gestational stages](/preview/png/2789004.png)
The concept of a network within the family of adhesion/growth regulatory galectins implies distinct spatio-temporal expression profiles. To test this assumption immunohistochemically for bovine placenta, placentomal (P) and interplacentomal tissues (IP) were collected at a slaughterhouse from the three stages of pregnancy (early gestation = day 30–130; mid gestation = day 130–220; late gestation = day 220–275). The specimens were snap-frozen or fixed in Bouin’s solution, then embedded in paraffin. Gene expression for galectins-1, -3, -4 and -9 in P and IP of late gestational stages was monitored by RT-PCR. Galectin-type-specific antibodies were used for immunohistochemical localization. In IP, galectin-1 was present in stroma cells and early gestational trophoblast giant cells (TGC), whereas galectin-3 was confined to uterine epithelial cells. In contrast, both galectins were found in epithelia of P tissue. Uterine epithelial cells and blood vessel walls were positive for galectin-4, while galectin-9 was detected predominantly in uterine epithelial cells and late gestational TGC. Our study thus reveals individual profiles among the galectins tested, an indication for specific functions exerted by each protein in the bovine endometrium and placenta.
Journal: Placenta - Volume 33, Issue 3, March 2012, Pages 195–201