کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2790157 1568581 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Placental NAD(P)H Oxidase Mediated Superoxide Generation in Early Pregnancy
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی تکاملی
پیش نمایش صفحه اول مقاله
Placental NAD(P)H Oxidase Mediated Superoxide Generation in Early Pregnancy
چکیده انگلیسی

Early placental development is characterised by rapid cell differentiation and migration, matrix remodelling and angiogenesis. The enzyme NAD(P)H oxidase is a major source of superoxide anions implicated in signalling pathways regulating these processes in other systems. It is also thought to be involved in oxygen sensing and regulation of the expression of antioxidant genes. We therefore investigated NAD(P)H oxidase activity in placental tissues in early pregnancy and at term, and correlated this with antioxidant capacity. We collected placental tissues from women undergoing termination of pregnancy (n = 19; gestational age 11+6 ± 1+0 weeks), and those with elective caesarean section at term after uncomplicated pregnancy (n = 15; gestational age 38+6 ± 0+4 weeks). Tissues were assayed for superoxide production, using lucigenin chemiluminescence, and three independent markers of antioxidant capacity. In human placentas from normal deliveries at term substantial basal NAD(P)H activity was present. Activity was almost threefold higher in early pregnancy (P < 0.0001). This was paralleled by higher total antioxidant capacity (P < 0.0001), tissue glutathione concentrations (P < 0.01) and gluthathione S-transferase enzyme activity (P < 0.05) when compared to corresponding term placental values. NAD(P)H oxidase mediated superoxide generation could be an important modulator of the antioxidant defence response in early pregnancy.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Placenta - Volume 27, Issues 2–3, February–March 2006, Pages 158–163
نویسندگان
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