Invariant natural killer T cells generated from human adult hematopoietic stem-progenitor cells are poly-functional

• iNKT cells can be generated from CD34+ stem progenitor cells using the OP9-DL1 system in combination with aAPC.
• iNKT cells cell be generated from bone marrow HSPC from lymphoma patients.
• HSPC-derived NKT cells produce IFN-γ, IL-4 and GM-CSF.

Invariant natural killer T (iNKT) cells constitute an important subset of T cells that can both directly and indirectly mediate anti-tumor immunity. However, cancer patients have a reduction in both iNKT cell number and function, and these deficits limit the potential clinical application of iNKT cells for cancer therapy. To overcome the problem of limited iNKT cell numbers, we investigated whether iNKT cells can be generated in vitro from bone marrow-derived adult hematopoietic stem-progenitor cells (HSPC). Our data demonstrate that co-culture of HSPC with OP9-DL1 stromal cells, results in a functional CD3+ T cell population. These T cells can be further differentiated into iNKT cells by secondary culture with CD1d-Ig-based artificial antigen-presenting cells (aAPC). Importantly, these in vitro-generated iNKT cells are functional, as demonstrated by their ability to proliferate and secrete IFN-γ and GM-CSF following stimulation.

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